Thymosin Alpha-1 5mg Research Peptide

Thymosin Alpha-1 5mg is a research peptide material associated with Thymosin Alpha-1 (Tα1), also known by the name thymalfasin. It is a synthetic, N-terminally acetylated peptide consisting of 28 amino-acid residues.

For laboratory researchers, Thymosin Alpha-1 is particularly relevant to peptide characterization, analytical chemistry, molecular identification, chromatographic testing, and research involving peptide structure and biological signaling.

The compound listed by PubChem as Thymalfasin has the molecular formula C129H215N33O55, a molecular weight of approximately 3,108.3 g/mol, and CAS Registry Number 62304-98-7. PubChem lists the sequence as SDAAVDTSSEITTKDLKEKKEVVEEAEN, with an N-terminal acetyl modification.

The 5mg designation refers to the quantity of material supplied and should not be confused with molecular weight, concentration, or analytical purity.

This page is designed to provide an evidence-based overview of Thymosin Alpha-1 5mg, including chemical identity, molecular weight, sequence, purity, HPLC analysis, COA documentation, analytical considerations, and research-use information.

Research-use statement: Thymosin Alpha-1 5mg discussed on this page is presented for laboratory research and analytical purposes only. It is not intended for human or veterinary administration, self-treatment, diagnosis, or prevention or treatment of disease.

What Is Thymosin Alpha-1?

Thymosin Alpha-1 is a 28-amino-acid peptide originally characterized from thymic material. Early structural research established that the peptide contains 28 amino-acid residues and has an acetylated serine at its N-terminus.

Modern chemical databases identify the compound as thymalfasin. PubChem describes thymalfasin as a chemically synthesized form of Thymosin Alpha-1 and provides its chemical formula, molecular weight, sequence, and structural identifiers.

Unlike small dipeptides such as Thymogen, Thymosin Alpha-1 is a substantially larger peptide. This difference is important when comparing research materials because the two compounds have different sequences, molecular formulas, molecular weights, and analytical profiles.

The peptide’s sequence is:

SDAAVDTSSEITTKDLKEKKEVVEEAEN

The biologically relevant structure includes an N-terminal acetyl group, commonly represented as:

Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH

PubChem lists this modified sequence and identifies Thymalfasin as an acetylated polypeptide.

Thymosin Alpha-1 5mg Key Specifications

Property	Information
Product	Thymosin Alpha-1 5mg
Common name	Thymosin Alpha-1
Synonym	Thymalfasin
Abbreviation	Tα1 / TA1
Peptide length	28 amino acids
Sequence	SDAAVDTSSEITTKDLKEKKEVVEEAEN
N-terminal modification	Acetylated
Molecular formula	C129H215N33O55
Molecular weight	Approximately 3,108.3 g/mol
CAS Registry Number	62304-98-7
Physical research material	Typically supplied as a lyophilized peptide where specified
Intended context	Laboratory research and analytical use

The chemical information above corresponds to the parent Thymalfasin record in PubChem.

Researchers should always verify the specific product specification and batch documentation supplied with the material.

Thymosin Alpha-1 Molecular Weight

, is approximately 3,108.3 g/mol. The molecular formula is C129H215N33O55.

Molecular weight is an important reference point for analytical research because it helps researchers evaluate expected mass-spectrometry signals, calculate molecular quantities, compare reference data, and distinguish Thymosin Alpha-1 from related peptides.

It is important to distinguish between:

Molecular weight
Average molecular mass
Exact or monoisotopic mass
Observed mass-to-charge ratio
Protonated or adducted MS ions

A mass-spectrometry result should therefore not be evaluated simply by comparing one observed m/z value with the molecular weight. Ionization state, charge state, adduct formation, instrument settings, and sample preparation can all affect the observed spectrum.

Molecular Weight vs. 5mg Quantity

The terms 3,108.3 g/mol and 5mg describe completely different properties.

The molecular weight describes the mass of one mole of the compound.

The 5mg specification describes the nominal quantity of material in the product.

Keeping these concepts separate is important when preparing technical product documentation and laboratory records.

Thymosin Alpha-1 Peptide Sequence

The established Thymosin Alpha-1 sequence is:

SDAAVDTSSEITTKDLKEKKEVVEEAEN

PubChem identifies this as the sequence for Thymalfasin and specifies the N-terminal acetylation in the structural representation.

The sequence contains 28 amino-acid residues and includes acidic and basic residues distributed throughout the peptide.

Historical structural research confirmed the primary structure using multiple analytical techniques, including sequence analysis and mass-spectrometric approaches.

For contemporary laboratory research, sequence information can be useful for:

Reference-standard comparison
Peptide identification
LC-MS method development
Analytical database searches
Structural characterization
Synthetic peptide verification

When comparing suppliers or batches, researchers should verify not only the amino-acid sequence but also relevant modifications. For Thymosin Alpha-1, the N-terminal acetylation is an important part of the molecular identity.

Thymosin Alpha-1 Purity

Purity is one of the most important analytical characteristics to review when evaluating a research peptide.

A product described as Thymosin Alpha-1 5mg should ideally have analytical documentation connecting the stated purity to a specific lot or batch number.

A generic statement such as “high purity” provides considerably less information than a batch-specific analytical report showing the method, result, specification, and sample identification.

What Does Peptide Purity Mean?

For peptide materials, purity is generally an analytical measurement reflecting the proportion of the detected target-related material under a defined testing method.

Different analytical methods can measure different aspects of a sample.

For example:

RP-HPLC can assess chromatographic purity.
LC-MS/MS can provide molecular identification information.
Mass spectrometry can help verify expected molecular mass.
Additional analytical methods can evaluate other attributes such as water, residual solvents, or counterions when applicable.

Therefore, a purity percentage should always be interpreted in the context of the analytical method used.

Why Batch-Specific Results Matter

The purity of one manufacturing batch should not automatically be represented as the purity of every future batch.

For example, a supplier may publish a COA showing a particular HPLC result for one lot. That result supports that particular lot and should not be copied onto another product page as a permanent specification unless the supplier’s documented specification supports that claim.

For SEO and scientific accuracy, the preferred wording is:

“Purity: see current batch-specific COA.”

This is more defensible than presenting an unsupported fixed percentage.

Thymosin Alpha-1 HPLC Analysis

High-performance liquid chromatography (HPLC) is commonly used for analytical characterization of peptide materials.

For Thymosin Alpha-1, reverse-phase HPLC can provide chromatographic separation between the principal peptide component and detectable related substances.

A typical HPLC report may contain:

Product name
Batch number
Sample identification
Column specification
Mobile-phase information
Detection wavelength
Retention time
Principal peak
Related peaks
Area percentage
Calculated purity
Test date
Laboratory identification

Reviewing a Thymosin Alpha-1 HPLC Chromatogram

When reviewing an HPLC chromatogram, researchers can first confirm that the product name and batch number correspond to the physical sample.

The principal chromatographic peak can then be evaluated along with any additional detectable peaks.

A reported HPLC purity percentage is generally calculated from chromatographic response under the specified method.

However, HPLC should not be treated as a complete identity test by itself.

A chromatogram can demonstrate that a major component behaves consistently with the expected analytical method, but additional characterization may be appropriate to establish molecular identity.

HPLC and Mass Spectrometry Together

For peptide characterization, HPLC and mass spectrometry can provide complementary information.

HPLC addresses chromatographic separation.

Mass spectrometry provides information about molecular mass and potentially fragmentation patterns.

Using both approaches can provide a more informative analytical profile than relying on either method alone.

Thymosin Alpha-1 COA

A Certificate of Analysis (COA) is an important quality document for a research peptide.

A well-structured Thymosin Alpha-1 5mg COA should connect the analytical results to the exact batch supplied.

Recommended COA Information

A comprehensive COA may contain:

Product name
Catalog number
Batch/lot number
CAS number
Molecular formula
Molecular weight
Peptide sequence
Appearance
HPLC purity
Identity test
Mass-spectrometry result
Manufacturing date
Testing date
Specification
Actual result
Storage information
Testing laboratory
Authorized approval

COA and Product Traceability

Batch traceability is particularly valuable for scientific research.

If a researcher records the batch number in an experimental notebook, the analytical documentation can later be connected to the material used in the experiment.

This can improve:

Reproducibility
Laboratory documentation
Quality control
Sample tracking
Supplier evaluation
Research recordkeeping

A product page should therefore encourage researchers to review the current COA rather than relying solely on a generic product description.

Thymosin Alpha-1 Identity Testing

Identity testing answers a different question from purity testing.

Purity asks: How much of the detected material corresponds to the principal chromatographic component under the stated method?

Identity asks: Does the material correspond to the intended chemical compound?

For Thymosin Alpha-1, identity evaluation may involve mass spectrometry or other suitable analytical methods.

PubChem identifies Thymalfasin using multiple chemical identifiers, including its sequence, molecular formula, and structural representations.

Historical research also used sequence analysis and mass spectrometry to confirm the primary structure of Thymosin Alpha-1.

This illustrates why modern peptide quality documentation can benefit from complementary analytical techniques.

Thymosin Alpha-1 Research Background

Thymosin Alpha-1 has been studied extensively in biomedical research.

A peer-reviewed review published in World Journal of Virology summarizes research involving Thymosin Alpha-1 and discusses its reported effects on immune-related processes and signaling.

Another review describes Thymosin Alpha-1 as a 28-amino-acid peptide and summarizes experimental research concerning T-cell, dendritic-cell, antibody, cytokine, and chemokine responses.

These publications provide useful scientific context for researchers, but research findings should not be converted into unsupported product claims.

For a research-product page, appropriate language focuses on:

Molecular identity
Peptide sequence
Analytical characterization
Research literature
Experimental context
Quality documentation

It should avoid claiming that a research product produces a particular therapeutic outcome in humans.

Research Applications of Thymosin Alpha-1

Thymosin Alpha-1 may be encountered in research involving peptide chemistry, analytical characterization, and studies of biological signaling.

Potential research areas include:

Peptide Characterization

Researchers can examine the chemical and structural properties of a defined 28-residue peptide.

Analytical Method Development

Thymosin Alpha-1 can be relevant to chromatographic and mass-spectrometric method-development workflows.

Peptide Identification

The known sequence and molecular formula provide reference information for analytical identification.

Structure-Activity Research

Researchers may investigate relationships between peptide structure, modifications, and experimentally observed biological activity.

Comparative Peptide Research

Thymosin Alpha-1 can be compared analytically with related thymic peptides or peptide analogues, provided the relevant controls and analytical methods are used.

Thymosin Alpha-1 5mg Quality Considerations

When evaluating a research peptide, several characteristics should be considered together.

1. Chemical Identity

The product should have a clearly defined identity and sequence.

2. Molecular Weight

The stated molecular weight should correspond to the correct molecular form, including relevant modifications.

3. Purity

Purity should be supported by an appropriate analytical method.

4. HPLC Documentation

The chromatogram should correspond to the relevant batch whenever supplied.

5. Mass-Spectrometry Data

Identity-supporting MS information can provide additional confidence in the reported molecular identity.

6. COA

A batch-specific Certificate of Analysis provides useful documentation and traceability.

7. Physical Description

Where applicable, the product should be clearly described regarding physical form and packaging.

8. Storage Documentation

Researchers should follow the supplier’s documented storage requirements and their institution’s applicable procedures.

Thymosin Alpha-1 5mg Research-Use Statement

Thymosin Alpha-1 5mg is presented strictly as a research-use-only laboratory material. It is intended for qualified scientific and analytical research and is not intended for human or veterinary administration, consumption, diagnosis, treatment, cure, or prevention of disease.

Product specifications should be verified using the current batch-specific documentation.

Literature describing Thymosin Alpha-1 biological activity represents findings from specific experimental or clinical research contexts and should not be interpreted as a guarantee of effects from a particular research material.

Frequently Asked Questions

What is Thymosin Alpha-1 5mg?

Thymosin Alpha-1 5mg refers to a product containing a nominal 5mg quantity of Thymosin Alpha-1, also known as thymalfasin. It is a 28-amino-acid, N-terminally acetylated peptide.

What is the molecular weight of Thymosin Alpha-1?

PubChem lists the molecular weight of Thymalfasin/Thymosin Alpha-1 as approximately 3,108.3 g/mol.

What is the molecular formula?

The molecular formula listed by PubChem is C129H215N33O55.

What is the Thymosin Alpha-1 sequence?

The 28-residue sequence is SDAAVDTSSEITTKDLKEKKEVVEEAEN, with an N-terminal acetyl modification.

What is Thymalfasin?

Thymalfasin is the name used for the chemically synthesized form of Thymosin Alpha-1. PubChem lists Thymalfasin and Thymosin Alpha-1 under the same chemical record.

Is Thymosin Alpha-1 the same as Thymogen?

No. Thymosin Alpha-1 is a 28-amino-acid peptide, whereas Thymogen is associated with the much smaller Glu-Trp dipeptide. They have different molecular structures and analytical properties.

What does Thymosin Alpha-1 HPLC purity mean?

It refers to the chromatographic purity calculated using the stated HPLC method. The reported value should be interpreted alongside the method and batch-specific analytical documentation.

What is a Thymosin Alpha-1 COA?

A COA is a Certificate of Analysis that documents analytical results for a particular batch or lot. It may include purity, identity, molecular information, appearance, and testing details.

Why should I check the batch-specific COA?

Because analytical results can vary between manufacturing batches. A batch-specific COA connects the reported result to the exact material being evaluated.

Is 5mg the molecular weight of Thymosin Alpha-1?

No. 5mg is the nominal product quantity. The molecular weight of Thymosin Alpha-1 is approximately 3,108.3 g/mol.

Is Thymosin Alpha-1 intended for human use on this page?

No. This page positions the material as research-use-only and does not provide instructions for human administration or self-use.