SS-31 10mg Research Peptide
SS-31 10mg is a laboratory research material associated with SS-31, elamipretide, and MTP-131, a synthetic mitochondria-targeting tetrapeptide studied extensively in mitochondrial biology.
SS-31 is structurally distinct from many conventional research peptides because it was designed with an aromatic-cationic sequence that enables interaction with mitochondrial membranes. Scientific literature describes elamipretide as a mitochondria-targeting tetrapeptide that interacts with cardiolipin, a phospholipid concentrated within the inner mitochondrial membrane.
For research-material buyers, however, the most important consideration is not simply the SS-31 name. Researchers should also evaluate identity, molecular formula, molecular weight, purity, HPLC data, mass-spectrometry confirmation, COA documentation, batch traceability, and storage information.
This guide explains the analytical and research characteristics of SS-31 10mg while keeping the material strictly within a laboratory research context.
What Is SS-31?
SS-31 is a synthetic Szeto-Schiller tetrapeptide also known as elamipretide and MTP-131.
A commonly reported SS-31 identity profile is:
| Property | SS-31 |
|---|---|
| Common name | SS-31 |
| Other names | Elamipretide, MTP-131 |
| Chemical class | Mitochondria-targeting tetrapeptide |
| CAS Number | 736992-21-5 |
| Molecular formula | C₃₂H₄₉N₉O₅ |
| Molecular weight | ~639.80 g/mol |
| Peptide length | 4 residues |
| Common research format | Lyophilized powder |
| Example research size | 10mg |
Current research-material documentation reports SS-31 as a four-residue peptide with a molecular formula of C₃₂H₄₉N₉O₅ and molecular weight around 639.8 g/mol.
The molecular identity should always be checked against the lot-specific certificate of analysis, because salt forms, formulation details, and supplier specifications can affect how a material is documented.
SS-31 Peptide Structure and Sequence
SS-31 is commonly represented as:
D-Arg-2′,6′-dimethyl-Tyr-Lys-Phe-NH₂
This sequence is important when distinguishing SS-31 from other mitochondrial research peptides.
Unlike a generic peptide described only by a product name, SS-31 has defined structural characteristics, including:
- D-arginine residue
- 2′,6′-dimethyltyrosine residue
- Lysine residue
- Phenylalanine residue
- C-terminal amide
Scientific publications describe SS-31/elamipretide as an aromatic-cationic tetrapeptide with the sequence D-Arg-2′6′-dimethylTyr-Lys-Phe-NH₂.
For analytical purposes, these structural characteristics provide useful reference points for identity testing.
SS-31 Molecular Weight
The commonly reported molecular weight of SS-31 is approximately:
639.80 g/mol
The corresponding molecular formula is:
C₃₂H₄₉N₉O₅
These values are useful when evaluating:
- Certificate of Analysis documents
- Mass spectrometry results
- Product specifications
- Peptide identity
- Analytical calculations
- Batch documentation
Current research-material documentation lists SS-31 at approximately 639.8 g/mol.
Researchers should distinguish the molecular weight of the peptide from the molecular weight of any particular salt or formulated material. A COA should make the relevant specification clear.
SS-31 10mg: What Does the 10mg Size Mean?
The 10mg designation refers to the stated quantity of SS-31 research material in the vial or package.
It does not independently establish:
- Purity
- Identity
- Peptide content
- Water content
- Residual solvents
- Stability
- HPLC performance
- Mass-spectrometry identity
For this reason, a 10mg research vial should be evaluated together with its analytical documentation.
A current SS-31 research listing, for example, provides a 10mg option and identifies the material as a lyophilized synthetic tetrapeptide.
The strongest product-page strategy is therefore to present 10mg quantity + analytical documentation + lot traceability, rather than using quantity as a substitute for quality information.
SS-31 Purity Testing
Why Is SS-31 Purity Important?
Purity testing provides information about the proportion of the analyzed material attributed to the target compound under a specified analytical method.
For synthetic peptides, RP-HPLC is commonly used to evaluate chromatographic purity.
A current SS-31 COA example reports:
HPLC purity: 99.163%
That result belongs to the specific lot identified on the certificate and should not be interpreted as a universal purity value for every SS-31 product.
Another current SS-31 research listing reports a batch HPLC purity of 99.0% for its specified lot.
These examples demonstrate why researchers should examine the actual COA for the specific batch rather than relying on a generic product-page claim.
SS-31 HPLC Analysis
High-performance liquid chromatography (HPLC) is one of the most useful analytical methods for evaluating peptide purity.
A typical RP-HPLC analysis can provide information such as:
- Retention time
- Main peptide peak
- Peak area
- Related peaks
- Chromatographic purity
- Analytical wavelength
- Column conditions
- Method parameters
For SS-31, an HPLC chromatogram can help researchers assess whether the target peptide represents the dominant chromatographic component under the stated method.
A strong SS-31 analytical package should ideally provide more than the statement:
“Purity >99%.”
Researchers should look for supporting details such as:
- HPLC method
- Detection wavelength
- Purity calculation
- Chromatogram
- Lot number
- Testing date
- Laboratory identification
This creates a more transparent connection between the numerical purity result and the underlying analytical evidence.
SS-31 Mass Spectrometry
Mass spectrometry provides an additional approach to peptide identity verification.
For SS-31, the expected molecular mass can be compared with an observed mass or mass-to-charge ratio generated by an appropriate MS method.
This can help distinguish SS-31 from unrelated peptide materials.
A useful analytical package may therefore combine:
RP-HPLC → purity
MS → molecular identity
The two methods answer different analytical questions.
HPLC evaluates chromatographic separation and purity, while mass spectrometry provides mass-based information supporting identity.
Researchers should also understand that molecular weight is not necessarily numerically identical to an observed MS m/z value. Ionization state and charge affect the measured mass-to-charge ratio.
SS-31 COA: Certificate of Analysis
A Certificate of Analysis (COA) is an important document for evaluating research material.
A useful SS-31 COA can include:
- Product name
- Alternative name
- CAS number
- Molecular formula
- Molecular weight
- Lot number
- Manufacturing date
- Testing date
- HPLC purity
- MS identity
- Peptide content
- Appearance
- Storage conditions
- Specification
- Actual result
- Pass/fail status
One current SS-31 COA example identifies the product as SS-31/elamipretide, gives CAS 736992-21-5, molecular weight 639.8 g/mol, formula C₃₂H₄₉N₉O₅, a 10mg claim, and an RP-HPLC purity result of 99.163%.
Match the COA to the Vial
The most important documentation check is:
Vial lot number = COA lot number
A COA from another batch should not automatically be treated as evidence for the material in a different vial.
SS-31 Analytical Testing
A comprehensive SS-31 analytical testing program may include multiple techniques.
RP-HPLC
Used primarily for chromatographic purity.
LC-MS or ESI-MS
Used to support molecular identity.
Mass Spectrometry
Used to evaluate molecular mass and characteristic peptide ions.
NMR
Can provide structural information using a different analytical principle.
Water Content
Can be evaluated where appropriate using a validated moisture-analysis method.
Residual Solvents
May be evaluated where relevant to manufacturing and purification processes.
Peptide Content
May be separately determined from chromatographic purity depending on the analytical specification.
The precise testing panel depends on the supplier, laboratory, intended research application, and quality-control specification.
SS-31 and Mitochondrial Research
SS-31 has attracted substantial scientific interest because of its interaction with mitochondrial membranes and cardiolipin.
Scientific reviews describe elamipretide as a mitochondria-targeting tetrapeptide that selectively interacts with cardiolipin in the inner mitochondrial membrane. Research has investigated how this interaction relates to mitochondrial membrane organization, bioenergetics, and oxidative processes.
Cardiolipin is a specialized phospholipid associated with the inner mitochondrial membrane and plays important structural and functional roles in mitochondrial biology.
Research involving SS-31 has therefore included areas such as:
- Mitochondrial membrane biology
- Cardiolipin interactions
- Mitochondrial bioenergetics
- Oxidative-stress mechanisms
- Electron-transport-chain research
- Cellular metabolism
- Mitochondrial structure
- Experimental models of mitochondrial dysfunction
These are research topics, not claims that an SS-31 research product is a treatment for any disease.
How SS-31 Interacts With Cardiolipin
One of the defining research characteristics of SS-31 is its interaction with cardiolipin.
Published research describes elamipretide as a mitochondria-targeting peptide that binds cardiolipin through electrostatic and hydrophobic interactions.
This interaction has made SS-31 useful in experimental studies investigating:
- Inner mitochondrial membrane organization
- Cardiolipin-dependent protein complexes
- Mitochondrial respiration
- Membrane electrostatic properties
- Cellular energy metabolism
A 2025 review notes that subsequent research has expanded understanding of elamipretide beyond early descriptions of reactive-oxygen-species effects, including its influence on mitochondrial membrane properties and cardiolipin-dependent protein organization.
SS-31 Research Applications
SS-31 research has been explored in numerous experimental settings.
Examples include:
Mitochondrial Biology
Researchers can use SS-31 as a tool compound when studying mitochondrial structure and function.
Cardiolipin Research
Because of its relationship with cardiolipin, SS-31 is relevant to experimental investigations of mitochondrial membrane lipids.
Cellular Bioenergetics
Studies have examined relationships between SS-31 and mitochondrial bioenergetic processes.
Oxidative-Stress Research
Experimental literature has examined SS-31 in models involving oxidative stress and mitochondrial function.
Mitochondrial Disease Models
Elamipretide has been investigated in preclinical and clinical research involving mitochondrial disorders.
These applications should be understood as scientific research areas, not instructions or recommendations for human use.
SS-31 Storage and Handling
SS-31 is commonly supplied as a lyophilized material.
A current research listing specifies storage of its lyophilized SS-31 at −20°C, protected from light and moisture.
Storage requirements can differ according to:
- Supplier
- Salt/formulation
- Packaging
- Concentration
- Stability data
- Whether the material is lyophilized or in solution
Researchers should therefore follow the storage instructions supplied with the specific batch and COA.
For laboratory inventory control, useful documentation includes:
- Date received
- Lot number
- Storage location
- Product quantity
- COA
- Retest/expiration information where provided
SS-31 Quality-Control Checklist
Before purchasing or evaluating SS-31 10mg research material, researchers can review the following checklist.
Identity
Confirm:
SS-31 / Elamipretide / MTP-131
CAS Number
Confirm:
736992-21-5
Molecular Formula
Confirm:
C₃₂H₄₉N₉O₅
Molecular Weight
Confirm:
Approximately 639.80 g/mol
HPLC
Review:
- Purity percentage
- Chromatogram
- Method
- Retention time
- Lot number
Mass Spectrometry
Review:
- Expected mass
- Observed mass
- Ionization information
- Identity interpretation
COA
Verify:
Product → Lot → COA
Storage
Check the specific supplier’s documented storage conditions.
This systematic approach provides more useful quality information than relying on a single purity percentage.