Introduction: What Is the MOTS-C Exact Mass?

MOTS-C is a 16-amino-acid mitochondrial-derived peptide that has become an important subject of biochemical, cellular, mitochondrial, and metabolic research. For laboratories working with synthetic peptides, analytical characterization is particularly important because molecular identity should be established using multiple complementary parameters rather than a single number.

The MOTS-C exact mass is 2174.1110958 Da, according to PubChem. PubChem also lists a molecular weight of 2174.6 g/mol and a monoisotopic mass of 2173.1077409 Da for its MOTS-C records. The associated molecular formula is C101H152N28O22S2.

The commonly listed human MOTS-C sequence is:

MRWQEMGYIFYPRKLR

This represents a 16-amino-acid peptide. PubChem identifies MOTS-C with CAS number 1627580-64-6 in its database records.

For researchers, understanding the difference between exact mass, molecular weight, monoisotopic mass, and experimentally observed LC-MS mass is essential. These values are related, but they are not interchangeable.

MOTS-C Exact Mass at a Glance

Property	MOTS-C information
Focus keyword	MOTS-C exact mass
Peptide	MOTS-C
Length	16 amino acids
Sequence	MRWQEMGYIFYPRKLR
Molecular formula	C101H152N28O22S2
Molecular weight	2174.6 g/mol
Exact mass	2174.1110958 Da
Monoisotopic mass	2173.1077409 Da
CAS	1627580-64-6
Research applications	Biochemical, analytical, cellular and mitochondrial research
Common analytical methods	HPLC, LC-MS/MS, mass spectrometry

The numerical values above are database-derived chemical properties and should be distinguished from the measured mass of a particular laboratory sample or salt form.

What Is the Exact Mass of MOTS-C?

The exact mass of MOTS-C is 2174.1110958 Da in the PubChem record. Exact mass refers to the calculated mass of a molecule using the masses of its constituent isotopes at their specified exact values.

For peptide researchers, exact mass can be particularly useful when evaluating high-resolution mass-spectrometry data.

It is important, however, not to confuse exact mass with molecular weight.

PubChem lists:

MOTS-C exact mass: 2174.1110958 Da
MOTS-C molecular weight: 2174.6 g/mol
MOTS-C monoisotopic mass: 2173.1077409 Da

These values appear together in PubChem’s chemical-property records and are useful for different analytical purposes.

Why Exact Mass Matters

When a laboratory analyzes a synthetic peptide, mass spectrometry can provide information about whether the detected molecular species is consistent with the expected molecular composition.

An expected mass can help researchers investigate:

Molecular identity
MOTS-C exact mass
Peptide composition
Potential modifications
Analytical impurities
Degradation products
Batch-to-batch differences
Unexpected molecular species

Mass data should normally be considered alongside chromatographic purity and other analytical documentation.

MOTS-C Molecular Weight vs. Exact Mass

One of the most common sources of confusion in peptide research is treating molecular weight and exact mass as the same measurement.

They are related, but technically different.

MOTS-C Molecular Weight

PubChem reports the molecular weight of MOTS-C as approximately:

2174.6 g/mol

Molecular weight is commonly used for general chemical identification, scientific documentation, molecular calculations, and product specifications.

MOTS-C Exact Mass

The exact mass is:

2174.1110958 Da

This value represents a more precise calculated molecular mass based on the elemental composition of the molecular species.

MOTS-C Monoisotopic Mass

PubChem reports:

2173.1077409 Da

The monoisotopic mass is associated with a defined isotopic composition and is particularly relevant when interpreting high-resolution mass spectrometry.

Why the Numbers Differ

A researcher may encounter all three values in technical literature or analytical software:

2174.6 g/mol ≠ 2174.1110958 Da ≠ 2173.1077409 Da

They describe related but different chemical-mass concepts.

For SEO and scientific documentation, using the correct terminology is important. A page targeting “MOTS-C exact mass” should not simply replace the exact-mass value with the molecular-weight value.

MOTS-C Molecular Formula

The molecular formula associated with MOTS-C in PubChem is:

C101H152N28O22S2

This formula indicates that the peptide contains:

101 carbon atoms
152 hydrogen atoms
28 nitrogen atoms
22 oxygen atoms
2 sulfur atoms

The elemental composition is important because the expected molecular mass is derived from the molecular composition.

Researchers comparing an expected peptide with LC-MS or HRMS data can use the formula and calculated mass as complementary identity parameters.

MOTS-C Peptide Sequence

The commonly represented human MOTS-C sequence is:

MRWQEMGYIFYPRKLR

It contains 16 amino acids.

Breaking the sequence into individual residues:

M – R – W – Q – E – M – G – Y – I – F – Y – P – R – K – L – R

The sequence is important for peptide identity because molecular mass alone may not provide sufficient information to distinguish every possible structural or sequence-related variant.

PubChem’s MOTS-C record lists the sequence MRWQEMGYIFYPRKLR.

For laboratory documentation, a robust peptide identity record may therefore include:

Peptide name
Amino-acid sequence
Molecular formula
Calculated molecular weight
Exact mass
Monoisotopic mass
Analytical mass spectrum
HPLC chromatogram
Lot or batch identification

What Is MOTS-C?

MOTS-C stands for mitochondrial open reading frame of the 12S rRNA-c and belongs to a group of peptides known as mitochondrial-derived peptides.

The original 2015 study reported MOTS-C as a 16-amino-acid peptide encoded within the mitochondrial 12S rRNA region. The researchers investigated its role in metabolic regulation using experimental models.

Subsequent research has investigated MOTS-C in connection with mitochondrial signaling, cellular metabolism, skeletal muscle biology, metabolic stress, and other areas of experimental biology.

These findings provide a scientific basis for continued laboratory research, but experimental findings should not be interpreted as proof of an established clinical use.

MOTS-C and Mitochondrial Research

Mitochondria are cellular organelles involved in energy production and numerous signaling pathways.

MOTS-C is unusual because its genetic origin is associated with mitochondrial DNA rather than the conventional nuclear genome.

The original study described a short open reading frame within mitochondrial DNA encoding the peptide and investigated its relationship to metabolic homeostasis.

Later reviews have examined MOTS-C as a mitochondrial-derived signaling peptide and discussed experimental findings involving metabolic regulation and cellular responses to stress.

For research purposes, this makes MOTS-C relevant to areas such as:

Mitochondrial biology
Cellular signaling
Peptide chemistry
Metabolic research
Molecular biology
Analytical chemistry
Proteomics
Mass spectrometry
Experimental aging research

MOTS-C Exact Mass and Mass Spectrometry

Mass spectrometry is one of the most useful analytical approaches for confirming peptide molecular identity.

For MOTS-C, the theoretical exact mass provides an expected reference value that can be compared with experimental mass-spectrometry observations.

A laboratory may use LC-MS or related high-resolution methods to investigate whether a peptide sample produces a molecular-ion pattern consistent with the expected composition.

However, an observed MS result can depend on several factors, including:

Ionization conditions
Charge state
Instrument calibration
Sample preparation
Counterions
Adducts
Solvent conditions
Peptide modifications
Instrument resolution

Consequently, researchers should not treat a single observed peak as a complete purity assessment.

Why Charge State Matters

Peptides can produce multiply charged ions during electrospray ionization.

Instead of observing a single ion corresponding directly to the molecular mass, an LC-MS instrument may detect different charge states.

This is why peptide researchers commonly evaluate the mass spectrum together with the theoretical molecular mass.

MOTS-C HPLC and Purity Analysis

HPLC and mass spectrometry answer different analytical questions.

HPLC primarily provides chromatographic separation and can help assess the relative abundance of the principal peptide peak and other detectable components.

Mass spectrometry provides molecular-mass information that can support identity assessment.

For a research-grade MOTS-C sample, researchers may therefore review both:

HPLC Data

A certificate of analysis may provide:

HPLC method
Detection wavelength
Retention time
Main peak
Reported purity
Batch or lot number

Mass-Spectrometry Data

An analytical report may include:

Calculated mass
Observed mass
Charge state
Mass spectrum
Instrument method
Molecular-ion assignment

Together, chromatographic and mass-spectrometric data can provide a more informative analytical profile than either measurement alone.

MOTS-C COA: What Researchers Should Check

A Certificate of Analysis (COA) is an important document for laboratory researchers evaluating a peptide material.

A useful COA should connect the analytical results to a specific batch or lot.

For MOTS-C, researchers may look for:

Product name
Lot number
Batch number
Stated peptide quantity
HPLC purity
Mass-spectrometry result
Molecular identity
Test date
Analytical method
Laboratory or testing information
Storage information where applicable

A COA should be reviewed as a batch-specific document rather than assuming that one certificate represents every future lot.

COA + HPLC + MS

For peptide research, the strongest documentation generally combines several layers of information:

Identity → Mass → Chromatographic purity → Batch documentation

This approach is more informative than relying only on a product title or advertised purity percentage.

MOTS-C Purity vs. Exact Mass

Purity and exact mass are different analytical concepts.

A peptide may have an expected molecular mass while still containing detectable impurities.

Similarly, a high chromatographic purity result does not by itself establish every aspect of molecular identity.

This is why researchers commonly consider:

Sequence + Formula + Expected Mass + MS + HPLC + COA

as complementary information.

When evaluating a MOTS-C research material, researchers should review the actual analytical documentation associated with the specific batch whenever available.

Why Researchers Search for “MOTS-C Exact Mass”

The keyword MOTS-C exact mass reflects a highly specific scientific search intent.

Researchers, students, analytical laboratories, and scientific-content readers may search the phrase to find:

A theoretical mass for LC-MS analysis
A peptide molecular-weight reference
Molecular formula information
Sequence information
Monoisotopic mass
HPLC/LC-MS documentation
COA information
Peptide identity references

This makes the keyword useful for a technical SEO page because it directly matches a defined analytical question.

MOTS-C Exact Mass for Laboratory Research

MOTS-C can be discussed in the context of laboratory research involving:

Peptide Chemistry

Researchers can study peptide composition, synthesis, characterization, and molecular properties.

Analytical Chemistry

Exact mass and molecular formula can serve as reference information when evaluating analytical data.

Mass Spectrometry

Theoretical mass values can help researchers interpret peptide ion signals and charge-state distributions.

Mitochondrial Biology

MOTS-C is studied as a mitochondrial-derived signaling peptide.

Cellular Research

Published research has investigated cellular responses associated with MOTS-C under experimental conditions.

The original research and later reviews provide scientific context, while individual laboratories should evaluate primary literature and experimental data directly.

MOTS-C Research Peptide: What Makes Analytical Documentation Important?

Research peptides are analytical materials rather than ordinary consumer products.

For laboratories, product documentation can be important because researchers need to know exactly what material is being evaluated.

A useful research-peptide specification can include:

Peptide name: MOTS-C
Sequence: MRWQEMGYIFYPRKLR
Formula: C101H152N28O22S2
Molecular weight: 2174.6 g/mol
Exact mass: 2174.1110958 Da
Monoisotopic mass: 2173.1077409 Da

The values above are reference chemical properties from PubChem. A supplier’s actual material specification should be checked against its own batch-specific documentation.